West Nile Virus Antikörper

Produktnummer ABIN7794789

Der Maus Monoklonal Anti-West Nile Virus Antikörper wurde für ELISA und FACS validiert. Er ist geeignet, West Nile Virus in Proben von West Nile Virus (WNV) zu detektieren.

Kurzübersicht für West Nile Virus Antikörper (ABIN7794789)

Target: West Nile Virus (WNV)

Reaktivität: West Nile Virus (WNV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser West Nile Virus Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS)

Klon: WNV-99

Produktdetails anti-West Nile Virus Antikörper

Verwendungszweck: Anti-West Nile Virus (Clone: WNV-99)-Purified No Carrier Protein

Spezifität: WNV-99 activity is directed against the wing, flexible loop of NS1. During the screening phase and post characterization clone WNV-99 was found to bind to solid-phase recombinant WNV NS1 protein and to NS1 expressed on the surface of intact WNV-infected Vero cells. Plus, also engaged the hexameric form of NS1. WNV-99 was cross-reactive against Japanese Encephalitis Virus and Tick-borne Encephalitis Virus in a direct ELISA using recombinant NS1 proteins. Background West Nile Virus (WNV) is a mosquito-borne, enveloped, positive-stranded RNA flavivirus1. Flavivirus nonstructural protein NS1 has been proposed as an antibody target to avoid antibody-dependent enhancement2. NS1 is a 46-55 kDa glycoprotein that is expressed as a dimer on the cell surface and as a soluble hexamer in the extracellular space and in circulation during infection. WNV NS1 dimer consists of a ?-roll, wing, and ?-ladder.

Aufreinigung: Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Reinheit: >=90 % monomer by analytical SEC and SDS-Page

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/mL

Buffer: This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at <= -70°C.Avoid Repeated Freeze Thaw Cycles.

Details zu West Nile Virus

Target: West Nile Virus (WNV)

Andere Bezeichnung: West Nile virus

Substanzklasse: Virus